Which method is utilized to isolate and contain areas during remediation?

The method employed to isolate and contain areas during remediation is crucial for preventing the spread of contaminants and ensuring safety. Sealing with tape is often used to secure materials, such as polyethylene barriers, to prevent any potential breaches that could release airborne particles or pathogens into uncontaminated areas. This ensures thorough containment of the affected space.

Negative air pressure is another essential technique in isolation, as it works by drawing air out of the contaminated area. This creates a pressure differential that minimizes the risk of airborne contaminants exiting the work area, thus protecting surrounding environments and personnel.

Polyethylene barriers provide a physical barrier between the contaminated area and the outside environment. They are often used in conjunction with the other methods to create secure, isolated zones where remediation work can occur without the risk of cross-contamination.

Therefore, the option incorporating all of these techniques indicates a comprehensive approach to ensuring safe and effective microbial remediation. Each method serves a specific purpose, and when combined, they enhance the overall effectiveness of containment efforts during the remediation process.